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Numéro d'article:
71679097
DMEM/F-12 Cell Culture Medium
Numéro d'article:
71679097
CAD 57
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DMEM/F-12 is widely used for supporting the growth of many different mammalian cells
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Ce qui se démarque
Versatile Formula
Combines DMEM and F-12 nutrients, supporting a wide range of cell types for various research applications, from adherent cells to suspension cultures.
Optimized Growth
Enhanced recipe fosters superior cell proliferation and maintenance, addressing common issues faced by researchers with slower growth rates in standard media.
Comprehensive Nutrient Mix
Includes essential vitamins, amino acids, and salts, ensuring cells receive all necessary components for optimal health and functionality, setting it apart from many competitor products.
Détails du produit
- DMEM/F-12 is a 1:1 mixture of DMEM and Ham’s F-12, containing amino acids, vitamins, inorganic salts, and other components necessary for multi-type cell culture.
- Widely used for supporting the growth of various mammalian cells, including MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.
- Does not contain proteins, lipids, or growth factors, hence supplementation with 10% Fetal Bovine Serum (FBS) is common.
- Requires a 5–10% CO2 environment to maintain physiological pH due to its sodium bicarbonate buffer system.
- Manufactured at a cGMP-compliant facility, using strict quality control testing for raw material components, and undergoes rigorous quality checks including sterility, pH, osmolality, endotoxin, and cell culture tests.
- Hygia cell culture media are produced using water for injection, filtered through 0.1 μm sterile filtration and aseptically filled in a Hundred-stage FFU with integrity tests being conducted before and after filtration.
| Date First Available | January 31, 2023 |
| Manufacturer | Hygia Reagents |
| Brand Name | Hygia Reagents |
| UPC | 755457565786 |
| Part Number | HCM-DMEM |
| Unit Count | 1.00 Count |
| Included Components | 1 bottle |
| Item Weight | 2 lbs (910 grams) |
À qui est-ce destiné ?
-
Cell Biologists
Ideal for researchers studying various cell types in vitro, supporting diverse growth and differentiation protocols.
-
Tissue Engineering
Suitable for applications in tissue engineering where cell growth and maintenance are critical for developing artificial tissues.
-
Pharmaceutical Research
Beneficial for drug discovery and testing, providing a stable environment for human and animal cells during experimentation.
-
Plant Cell Cultures
Not suitable for plant cell cultures which require specific nutrients and growth factors not found in DMEM/F-12.
DESCRIPTION DU PRODUIT
DMEM/F-12 Cell Culture Medium
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Questions et réponses des clients
-
question:
What is DMEMF-12 Cell Culture Medium used for?
répondre: DMEMF-12 Cell Culture Medium is primarily used for the growth and maintenance of mammalian cells in laboratory settings. It is specifically formulated to support the nutritional needs of fast-growing cells, providing essential amino acids, vitamins, and minerals. Researchers commonly utilize this medium in experiments involving cell proliferation, genetic engineering, and drug development, ensuring optimal conditions for cell viability and functionality. -
question:
How do you prepare DMEMF-12 Cell Culture Medium?
répondre: Preparing DMEMF-12 Cell Culture Medium involves reconstituting the powdered form with sterile distilled water or a suitable buffer. It is important to adjust the pH to around 7.2-7.4, which provides the necessary environment for cell growth. Additionally, supplementing the medium with fetal bovine serum or other growth factors enhances its effectiveness. This preparation is crucial as it ensures that the cells receive the right nutrients and maintain their health during experiments. -
question:
Can DMEMF-12 be used for primary cell cultures?
répondre: Yes, DMEMF-12 Cell Culture Medium can be utilized for primary cell cultures, particularly for a variety of mammalian cells. Its formulation is designed to cater to the growth requirements of both established cell lines and primary cells. Its versatility makes it ideal for researchers conducting studies on tissue regeneration, cellular response, and metabolic processes in a closer-to-native environment. -
question:
What makes DMEMF-12 different from other culture media?
répondre: DMEMF-12 differentiates itself from other culture media by its unique blend of nutrients tailored for fast-growing cells. Its composition allows for better cell attachment, proliferation, and metabolic activity compared to standard DMEM. Researchers select DMEMF-12 for high-cell-density applications, ensuring improved experimental outcomes in studies related to cancer research, regenerative medicine, and pharmacological testing. -
question:
Is DMEMF-12 suitable for serum-free culture?
répondre: DMEMF-12 can be adapted for serum-free culture by supplementing it with specific growth factors and hormones tailored to the cell line being used. This serum-free approach minimizes variability and allows for a more defined environment. Many researchers prefer serum-free conditions for regulatory and consistency reasons, especially when conducting assays or scales relevant to biotech applications. -
question:
How should DMEMF-12 Cell Culture Medium be stored?
répondre: DMEMF-12 Cell Culture Medium should be stored in a cool, dry place away from direct sunlight. Once prepared, it is best to store the medium at 2-8 degrees Celsius for short-term use or freeze it for long-term storage. Proper storage is essential to maintain its integrity and effectiveness, ensuring that the cells receive the necessary nutrients and conditions for optimal growth. -
question:
What cell lines are commonly grown in DMEMF-12?
répondre: Commonly used cell lines such as HEK293, CHO, and various cancer cell lines thrive in DMEMF-12 Cell Culture Medium. These cell lines are often employed in genetic, pharmaceutical, and toxicological studies. Researchers choose DMEMF-12 to support robust growth and high cell yield, which is crucial for experiments requiring significant cell quantities for analysis. -
question:
Can DMEMF-12 be modified or supplemented?
répondre: DMEMF-12 can be modified or supplemented to better suit specific cell growth needs. Researchers might add components such as L-glutamine, non-essential amino acids, or specialized growth factors depending on the cell type. Customization allows for greater control over growth conditions, enabling researchers to optimize their cultures for experiments related to gene expression, cell signaling, and drug response. -
question:
What are the signs of spoiled DMEMF-12 Medium?
répondre: Signs of spoiled DMEMF-12 Medium include changes in color, presence of precipitate, or an unusual odor. Additionally, if cell cultures show poor growth or abnormal morphology after media change, it may indicate spoilage. For consistent and reproducible results, it is imperative to routinely check the medium for these indicators and replace it when necessary to maintain cell health and experimental accuracy. -
question:
Where can I buy DMEMF-12 Cell Culture Medium in Canada?
répondre: You can purchase DMEMF-12 Cell Culture Medium through Ubuy in Canada. Ubuy offers a wide range of laboratory supplies and ensures that you can find the specific formulations you need for your research. With various products available, Ubuy serves as a convenient platform for researchers looking to acquire high-quality cell culture media.
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Avantages
- High nutrient content
- Supports a variety of cell types
- Reliable for long-term cultures
- Excellent pH stability over time
- Easy to prepare and use
Les inconvénients
- Occasional sediment formation
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Caractéristiques et avantages
- DMEM/F-12 is a 1:1 mixture of DMEM and Ham’s F-12
- It contains amino acids, vitamins, inorganic salts and other components needed for multi-type cell culture.
- DMEM/F-12 contains no proteins, lipids, or growth factors which requires additional supplementation.
- Hygia’s cell culture media products are screened through strict quality control testing.
- Manufactured at a cGMP-compliant facility using sterilized water.
- Each lot of DMEM/F-12 is subjected to multiple tests to ensure the highest quality.
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